the mir-383-ldha axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
Authors
abstract
objective(s): to explore the correlation between expression patterns and functions of mir-383 and ldha in hepatocellular cancer (hcc). materials and methods: we detected the expression of mir-383 and ldha in 30 hcc tissues and their matched adjacent normal tissues using qrt-pcr. then we performed mtt assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of mir-383 in cell proliferation, invasion and glycolysis in hcc cell lines. luciferase reporter assay was used to explore whether ldha was a target gene of mir-383. western blot and qrt-pcr were used to further confirm ldha was targeted by mir-383. then the above functional experiments were repeated to see whether the function of ldha could be inhibited by mir-383. results: the results of qrt-pcr showed that mir-383 was down-regulated in hcc tissues compared with their matched adjacent normal tissues. functional experiments showed that overexpression of mir-383 significantly suppressed cell proliferation, invasion and glycolysis. luciferase reporter assay showed ldha was a target gene of mir-383 and expression of ldha was inversely correlated with that of mir-383 in hcc. besides, increased cell proliferation, invasion and glycolysis triggered by ldha could be inhibited by overexpression of mir-383 in hcc cell lines. conclusion: our study proved that mir-383 is down-regulated in hcc and acts as a tumor suppressor through targeting ldha. targeting the mir-383-ldha axis might be a promising strategy in hcc treatment.
similar resources
The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...
full textThe miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
OBJECTIVES To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). MATERIALS AND METHODS We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate producti...
full textmiR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer
Objective(s): Ten-eleven translocation (TET) family members have been shown to be involved in the development of many tumors. However, the biological role of the TET family and its mechanism of action in colorectal carcinogenesis and progression remain poorly understood. Materials and Methods:We measured the expression levels of TET family members in colorectal cancer (CRC) specimens, in the c...
full textThe c-Myc–LDHA axis positively regulates aerobic glycolysis and promotes tumor progression in pancreatic cancer
The transcription factor c-Myc plays critical roles in cancer development and progression through regulating expression of targeted genes. Lactate dehydrogenase A (LDHA), which catalyzes the conversion of L-lactate to pyruvate in the final step of anaerobic glycolysis, is frequently upregulated in pancreatic cancer. However, little is known about the effects of c-Myc-LDHA axis in the progressio...
full textThe miR-34a-LDHA axis regulates glucose metabolism and tumor growth in breast cancer.
Lactate dehydrogenase A (LDHA) is involved in a variety of cancers. The purpose of this study was to investigate the expression, prognostic roles and function of LDHA in breast cancer. We found that LDHA was upregulated in both breast cancer cell lines and clinical specimens using quantitative real-time PCR (qRT-PCR). Immunohistochemistry (IHC) analysis of tissue microarrays (TMAs) showed that ...
full textmiR-92a promotes hepatocellular carcinoma cells proliferation and invasion by FOXA2 targeting
Objective(s): MicroRNAs (miRNAs) are considered as powerful, post-transcriptional regulators of gene expression in hepatocellular carcinoma cells (HCC). However, the function of miR-92a is still unclear in HCC. Materials and Methods: Expression of miR-92a in human HCC cell lines was evaluated using qRT-PCR. MTT assay and transwell assay were used to examine the function of miR-92a in HepG2 and ...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of basic medical sciencesجلد ۲۰، شماره ۲، صفحات ۱۸۷-۱۹۲
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023